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Accueil > Evénements > Séminaires > Archives 2015 > Zero-Mode Waveguide (...)
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Séminaire « Matière molle : organisation et dynamique »

Zero-Mode Waveguide Detection of Flow-Driven DNA Translocation through Nanopores - Fabien Montel - Lundi 4 mai à 11 h

INSP - 4 place Jussieu - 75252 PARIS Cedex 05 - Barre 22-32 - 2e étage, salle 201

Fabien Montel - Laboratoire Matière et Systèmes Complexes, Université Paris Diderot-CNRS

Résumé

Flow injection of polymers into nanopores is a critical step of many industrial processes including filtration and oil recovery. In our work, we directly monitor the flow driven injection of DNA through nanopores at the single molecule and single pore level using a custom zero mode wave guide method. We observe a flow threshold independent of the pore radius, the DNA concentration and length. We demonstrate that the flow injection of DNA in nanopores is controlled by an energy barrier as proposed by the de Gennes - Brochard suction model. Finally, we show that the height of the energy barrier can be modulated by functionalizing the nanopores with flexible polymers (PEG-thiol) and that the modulation is correlated to the length of the PEG polymers. With the experimental set up we have designed, we aim at exploring the dynamics of biopolymer transport through the nuclear pore complex as well as developing solid state membranes for smart-filtration.